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ATCC61240 Fr8-77
基本信息
资源编号 ATCC61240
资源名称 Fr8-77
种属 Homo sapiens, human
安全等级 1
培养方法
培养基 ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
详细说明
描述 Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; HindIII--4.4; EcoRI/HindIII--3.0, 1.45; BglI--4.4; PvuII--4.4.
Insert can be labeled by transcription with SP6 polymerase on HindIII linearized plasmid.
Codominant segregation shown in 2 informative families (10 individuals).
The locus D21S82 is very closely linked to the locus SOD1 on chromosome 21.
No background at stringency of 0.2X SSC, 65C.
Enzyme(s) not detecting polymorphism: BglII, HindIII, MspI, PstI, RsaI and TaqI, in 5 unrelated Caucasians.
Vector Construct size (kb) 4.5
Insert DNA genomic
Insert lengths(kb) 1.5
Gene product DNA Segment, single copy [D21S82]
Alleles A3, B2, A1, A2, B1, B3
Insert Size (kb) 1.5
Shipping Information Distributed freeze-dried
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