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ATCCPCS-400-010 Primary Renal Proximal Tubule Epitheli ..
基本信息
资源编号 ATCCPCS-400-010
资源名称 Primary Renal Proximal Tubule Epitheli ..
种属 Homo sapiens, human Kidney
类型 Epithelial cell
形态 Cuboidal with a characteristic pattern of swirled cells; refractile
提供形式 frozen 1 mL
致病类型 Normal
安全等级 1
应用领域 Applications for use might include research related to hypertension; diabetes; oncology; renal fibrosis; inflammation; autoimmune disease; drug screening/development; and toxicology.
培养方法
培养基 Obtain one Renal Epithelial Cell Growth Kit from the freezer; make sure that the caps of all components are tight. Thaw the components of the growth kit just prior to adding them to the basal medium. Obtain one bottle of Renal Epithelial Cell Basal Medium (485 mL) from cold storage. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.        Table 1. Renal Epithelial Cell Growth Kit Components 


Component

 



Volume

 



Final Concentration

 



Fetal Bovine Serum (FBS)

 



2.5 mL

 



0.5%

 



Triiodothyronine

 



0.5 mL

 



10 nM

 



rh EGF

 



1.0 mL

 



10 ng/mL

 



Hydrocortisone Hemisuccinate

 



0.5 mL

 



100 ng/mL

 



rh Insulin

 



0.5 mL

 



5 mg/ml

 



Epinephrine

 



0.5 mL

 



1.0 mM

 



Transferrin

 



0.5 mL

 



5 mg/ml

 



L-Alanyl-L-Glutamine

 



6.0 mL

 



2.4 mM

 


  Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of either of the optional components (GA solution or PSA solution) to be added to the complete growth media is summarized in Table 2.
  Table 2. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)


Component

 



Volume

 



Final Concentration

 

生长条件 Gentamicin-Amphotericin B Solution
0.5 mL
Gentamicin: 10 µg/mL
Amphotericin B: 0.25 µg/mL
Penicillin-Streptomycin-Amphotericin B Solution
0.5 mL
Penicillin: 10 Units/mL
Streptomycin: 10 µg/mL
Amphotericin B: 25 ng/mL
Phenol Red
0.5 mL
33 µM
生长特性 Adherent
详细说明
描述 A complete solution to propagate renal proximal tubule epithelial cells with excellent morphology in low serum (0.5% FBS) conditions.
Age Batch-specific
Gender Batch-specific
Ethnicity Batch-specific
Volume 1 mL
Cells per Vial Onevialcontainsaminimumof 5x105 viablecells.
Sterility Tests Bacteria and Yeast: Negative Mycoplasma: Negative
Viral Testing Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative
Viability ≥50% when thawed from cryopreservation
Population Doubling Capacity ≥15 in complete growth medium
C of A Certificate of Analysis
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